Composite

Part:BBa_K5366023:Design

Designed by: Lin Li   Group: iGEM24_NJTECH-CHINA-A   (2024-09-27)


T7 promoter-RBS-MBC-6xHis-T7 termonator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 262
    Illegal PstI site found at 283
    Illegal PstI site found at 658
    Illegal PstI site found at 700
    Illegal PstI site found at 910
    Illegal PstI site found at 1468
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1543
    Illegal PstI site found at 262
    Illegal PstI site found at 283
    Illegal PstI site found at 658
    Illegal PstI site found at 700
    Illegal PstI site found at 910
    Illegal PstI site found at 1468
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 262
    Illegal PstI site found at 283
    Illegal PstI site found at 658
    Illegal PstI site found at 700
    Illegal PstI site found at 910
    Illegal PstI site found at 1468
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 262
    Illegal PstI site found at 283
    Illegal PstI site found at 658
    Illegal PstI site found at 700
    Illegal PstI site found at 910
    Illegal PstI site found at 1468
    Illegal NgoMIV site found at 1117
    Illegal AgeI site found at 406
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

none


Source

Chloroflexota bacterium

References